Bacterio-opsin mutants of Halobacterium halobium
نویسندگان
چکیده
منابع مشابه
Bacterio-opsin mutants of Halobacterium halobium.
The bacterio-opsin (bop) gene of Halobacterium halobium R1 has been cloned with about 40 kilobases of flanking genomic sequence. The 40-kilobase segment is derived from the (G+C)-rich fraction of the chromosome and is not homologous to the major (pHH1) or minor endogenous covalently closed circular DNA species of H. halobium. A 5.1-kilobase Pst I fragment containing the bop gene was subcloned i...
متن کاملBacterio-opsin mRNA in wild-type and bacterio-opsin-deficient Halobacterium halobium strains.
We have examined transcripts corresponding to the Halobacterium halobium bacterio-opsin (BO) gene in wild-type and in BO-deficient mutant strains containing the insertion elements ISH1 or ISH2 in the BO gene. BO mRNA from the wild-type strain was purified by hybrid selection using single-stranded cDNA. Labeling by vaccinia virus capping enzyme and [alpha-(32)P]GTP showed that it contains the 5'...
متن کاملCharacterization of insertions affecting the expression of the bacterio-opsin gene in Halobacterium halobium.
We have determined the sequence of the inverted repeats and duplicated target DNA of the halobacterial insertion elements ISH2 (520 bp), ISH23 (900 bp) and ISH24 (3000 bp) associated with bacterio-opsin (bop) mutants. ISH2 has a perfect 19 bp inverted repeat (3,5), while both ISH23 and ISH24 have imperfect inverted repeats of 29 bp and 14 bp respectively. ISH23 was shown to be highly homologous...
متن کاملGenetic variability in Halobacterium halobium.
Halobacterium halobium exhibits an extraordinary degree of spontaneous variability. Mutants which are defective in the formation of gas vacuoles (vac) arise at a frequency of 10(-2). Other easily detectable phenotypes, like the synthesis of bacterioruberin (Rub) or the synthesis of retinal (Ret) and bacterio-opsin (Ops), the two components which form the purple membrane (Pum) of H. halobium, ar...
متن کاملIntegration of photosensory signals in Halobacterium halobium.
Stimulation of Halobacterium halobium through its sensory photosystems, PS 370 and PS 565, leads either to a prolonged or to a shortened interval between two reversals of the swimming direction of the cell, the attractant or repellent response. Stimuli are integrated to yield the same response regardless through which photosystem they are given. Simultaneously elicited attractant and repellent ...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1983
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.80.5.1416